Microsatellite Loci for an Old Rare Species, Pseudotaxus chienii, and Transferability in Taxus wallichiana var. mairei (Taxaceae)
نویسندگان
چکیده
منابع مشابه
Microsatellite loci for an old rare species, Pseudotaxus chienii, and transferability in Taxus wallichiana var. mairei (Taxaceae)1
UNLABELLED PREMISE OF THE STUDY Microsatellite loci were developed for Pseudotaxus chienii, an old rare species endemic to China, and which provided a useful tool for investigating the patterns of population genetic structure, phylogeography, evolutionary history, and adaptive potential. Transferability was assayed in the related species, Taxus wallichiana var. mairei. • METHODS AND RESULT...
متن کاملDevelopment of microsatellite loci for Taxus wallichiana var. wallichiana (Taxaceae) and cross-amplification in Taxaceae.
Nine polymorphic microsatellite loci were isolated and characterized in Taxus wallichiana var. wallichiana, an endangered species in China. The number of alleles per locus ranged from 2 to 20. Observed and expected heterozygosities varied from 0.0260 to 0.5325 and 0.3603 to 0.9231, respectively. Positive cross-amplification of the 9 loci was observed in 2 other varieties of T. wallichiana and 4...
متن کاملChloroplast microsatellite markers for Pseudotaxus chienii developed from the whole chloroplast genome of Taxus chinensis var. mairei (Taxaceae)1
PREMISE OF THE STUDY Pseudotaxus chienii (Taxaceae) is an old rare species endemic to China that has adapted well to ecological heterogeneity with high genetic diversity in its nuclear genome. However, the genetic variation in its chloroplast genome is unknown. METHODS AND RESULTS Eighteen chloroplast microsatellite markers (cpSSRs) were developed from the whole chloroplast genome of Taxus ch...
متن کاملDevelopment and characterization of microsatellite loci in the endangered species Taxus wallichiana (Taxaceae)1
UNLABELLED PREMISE OF THE STUDY Microsatellite primers were developed in the endangered tree species Taxus wallichiana from Nepal to investigate regional genetic differentiation, local genetic diversity, and gene flow for the conservation of this species under climate- and land-use change scenarios in mountain regions of Nepal. • METHODS AND RESULTS We developed 10 highly polymorphic micro...
متن کاملIsolation and Identification of an Endophytic Fungus Producing Paclitaxel from Taxus Wallichiana Var Mairei.
The objective of this study was to isolate endophytic fungi producing paclitaxel from yew for the purpose of paclitaxel manufacture. Surface sterilized bark of Taxus wallichiana var. mairei was used as source material and potato dextrose agar culture medium was used in isolation of endophytic fungi. Fungal cultures were extracted with a mixture of chloroform / methanol (1:1, v/v) and the paclit...
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ژورنال
عنوان ژورنال: Applications in Plant Sciences
سال: 2013
ISSN: 2168-0450
DOI: 10.3732/apps.1200456